|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat REG4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80249-ACG|
|Rat REG4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80249-ACR|
|Rat REG4 ORF mammalian expression plasmid, C-Flag tag||RG80249-CF|
|Rat REG4 ORF mammalian expression plasmid, C-His tag||RG80249-CH|
|Rat REG4 ORF mammalian expression plasmid, C-Myc tag||RG80249-CM|
|Rat REG4 ORF mammalian expression plasmid, C-HA tag||RG80249-CY|
|Rat REG4 Gene cDNA clone plasmid||RG80249-G|
|Rat REG4 ORF mammalian expression plasmid, N-Flag tag||RG80249-NF|
|Rat REG4 ORF mammalian expression plasmid, N-His tag||RG80249-NH|
|Rat REG4 ORF mammalian expression plasmid, N-Myc tag||RG80249-NM|
|Rat REG4 ORF mammalian expression plasmid, N-HA tag||RG80249-NY|
|Rat REG4 natural ORF mammalian expression plasmid||RG80249-UT|
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Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.