|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TCN2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10566-ACG|
|Human TCN2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10566-ACR|
|Human TCN2 ORF mammalian expression plasmid, C-Flag tag||HG10566-CF|
|Human TCN2 ORF mammalian expression plasmid, C-His tag||HG10566-CH|
|Human TCN2 ORF mammalian expression plasmid, C-Myc tag||HG10566-CM|
|Human TCN2 ORF mammalian expression plasmid, C-HA tag||HG10566-CY|
|Human TCN2 Gene cDNA clone plasmid||HG10566-M|
|Human TCN2 ORF mammalian expression plasmid, N-Flag tag||HG10566-NF|
|Human TCN2 ORF mammalian expression plasmid, N-His tag||HG10566-NH|
|Human TCN2 ORF mammalian expression plasmid, N-Myc tag||HG10566-NM|
|Human TCN2 ORF mammalian expression plasmid, N-HA tag||HG10566-NY|
|Human TCN2 natural ORF mammalian expression plasmid||HG10566-UT|
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Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.