|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLEC4F ORF mammalian expression plasmid, C-GFPSpark tag||RG80215-ACG|
|Rat CLEC4F ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80215-ACR|
|Rat CLEC4F ORF mammalian expression plasmid, C-Flag tag||RG80215-CF|
|Rat CLEC4F ORF mammalian expression plasmid, C-His tag||RG80215-CH|
|Rat CLEC4F ORF mammalian expression plasmid, C-Myc tag||RG80215-CM|
|Rat CLEC4F ORF mammalian expression plasmid, C-HA tag||RG80215-CY|
|Rat CLEC4F Gene cDNA clone plasmid||RG80215-G|
|Rat CLEC4F ORF mammalian expression plasmid, N-Flag tag||RG80215-NF|
|Rat CLEC4F ORF mammalian expression plasmid, N-His tag||RG80215-NH|
|Rat CLEC4F ORF mammalian expression plasmid, N-Myc tag||RG80215-NM|
|Rat CLEC4F ORF mammalian expression plasmid, N-HA tag||RG80215-NY|
|Rat CLEC4F natural ORF mammalian expression plasmid||RG80215-UT|
|Learn more about expression Vectors|
CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F（mCLEC4F） is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.