|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLEC4D ORF mammalian expression plasmid, C-GFPSpark tag||RG80213-ACG|
|Rat CLEC4D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80213-ACR|
|Rat CLEC4D ORF mammalian expression plasmid, C-Flag tag||RG80213-CF|
|Rat CLEC4D ORF mammalian expression plasmid, C-His tag||RG80213-CH|
|Rat CLEC4D ORF mammalian expression plasmid, C-Myc tag||RG80213-CM|
|Rat CLEC4D ORF mammalian expression plasmid, C-HA tag||RG80213-CY|
|Rat CLEC4D Gene cDNA clone plasmid||RG80213-G|
|Rat CLEC4D ORF mammalian expression plasmid, N-Flag tag||RG80213-NF|
|Rat CLEC4D ORF mammalian expression plasmid, N-His tag||RG80213-NH|
|Rat CLEC4D ORF mammalian expression plasmid, N-Myc tag||RG80213-NM|
|Rat CLEC4D ORF mammalian expression plasmid, N-HA tag||RG80213-NY|
|Rat CLEC4D natural ORF mammalian expression plasmid||RG80213-UT|
|Learn more about expression Vectors|
C-type lectin (CLEC) family is a type of carbohydrate-binding protein domain named lectin. C-type lectins are the most diverse and prevalent lectin family in immunity with its requirement for calcium for binding. Proteins including a C-type lectin domain have diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. There are at least 14 types of C-type lectins: typeⅠto typeⅩⅣ. CLEC4D(CLECSF8) is a typeⅡ membrane glycoprotein belonging to the C-type lectin family, with restricted expression in the monocyte/macrophage lineage. It plays important roles in the function of macrophages.