|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLEC10A ORF mammalian expression plasmid, C-GFPSpark tag||RG80210-ACG|
|Rat CLEC10A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80210-ACR|
|Rat CLEC10A ORF mammalian expression plasmid, C-Flag tag||RG80210-CF|
|Rat CLEC10A ORF mammalian expression plasmid, C-His tag||RG80210-CH|
|Rat CLEC10A ORF mammalian expression plasmid, C-Myc tag||RG80210-CM|
|Rat CLEC10A ORF mammalian expression plasmid, C-HA tag||RG80210-CY|
|Rat CLEC10A ORF mammalian expression plasmid, N-Flag tag||RG80210-NF|
|Rat CLEC10A ORF mammalian expression plasmid, N-His tag||RG80210-NH|
|Rat CLEC10A ORF mammalian expression plasmid, N-Myc tag||RG80210-NM|
|Rat CLEC10A ORF mammalian expression plasmid, N-HA tag||RG80210-NY|
|Rat CLEC10A Gene cDNA clone plasmid||RG80210-U|
|Rat CLEC10A natural ORF mammalian expression plasmid||RG80210-UT|
|Learn more about expression Vectors|
CLEC10A, also known as the macrophage galactose-type calcium-type lectins (MGLs; CD301) constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. MGLs/CD301 is a type II transmembrane glycoproteins and is expressed on macrophages and related cells of myeloid origins, particularly immature dendritic cells (DCs). There are 2 homologues: MGL1 and MGL2 (CD301a and CD301b) in mice. MGL1/CD301a induces both the production and secretion of interleukin (IL)-10. MGL1/CD301a plays a protective role against colitis by effectively inducing IL-10 production by colonic lamina propria macrophages in response to invading commensal bacteria.