|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL27 ORF mammalian expression plasmid, C-GFPSpark tag||RG80201-ACG|
|Rat IL27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80201-ACR|
|Rat IL27 ORF mammalian expression plasmid, C-Flag tag||RG80201-CF|
|Rat IL27 ORF mammalian expression plasmid, C-His tag||RG80201-CH|
|Rat IL27 ORF mammalian expression plasmid, C-Myc tag||RG80201-CM|
|Rat IL27 ORF mammalian expression plasmid, C-HA tag||RG80201-CY|
|Rat IL27 Gene cDNA clone plasmid||RG80201-G|
|Rat IL27 ORF mammalian expression plasmid, N-Flag tag||RG80201-NF|
|Rat IL27 ORF mammalian expression plasmid, N-His tag||RG80201-NH|
|Rat IL27 ORF mammalian expression plasmid, N-Myc tag||RG80201-NM|
|Rat IL27 ORF mammalian expression plasmid, N-HA tag||RG80201-NY|
|Rat IL27 natural ORF mammalian expression plasmid||RG80201-UT|
|Learn more about expression Vectors|
IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. IL-27 protein is also referred as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.