|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL4R ORF mammalian expression plasmid, C-GFPSpark tag||RG80198-ACG|
|Rat IL4R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80198-ACR|
|Rat IL4R ORF mammalian expression plasmid, C-Flag tag||RG80198-CF|
|Rat IL4R ORF mammalian expression plasmid, C-His tag||RG80198-CH|
|Rat IL4R ORF mammalian expression plasmid, C-Myc tag||RG80198-CM|
|Rat IL4R ORF mammalian expression plasmid, C-HA tag||RG80198-CY|
|Rat IL4R Gene cDNA clone plasmid||RG80198-G|
|Rat IL4R ORF mammalian expression plasmid, N-Flag tag||RG80198-NF|
|Rat IL4R ORF mammalian expression plasmid, N-His tag||RG80198-NH|
|Rat IL4R ORF mammalian expression plasmid, N-Myc tag||RG80198-NM|
|Rat IL4R ORF mammalian expression plasmid, N-HA tag||RG80198-NY|
|Rat IL4R natural ORF mammalian expression plasmid||RG80198-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.