|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL13RA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80186-ACG|
|Rat IL13RA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80186-ACR|
|Rat IL13RA1 ORF mammalian expression plasmid, C-Flag tag||RG80186-CF|
|Rat IL13RA1 ORF mammalian expression plasmid, C-His tag||RG80186-CH|
|Rat IL13RA1 ORF mammalian expression plasmid, C-Myc tag||RG80186-CM|
|Rat IL13RA1 ORF mammalian expression plasmid, C-HA tag||RG80186-CY|
|Rat IL13RA1 Gene cDNA clone plasmid||RG80186-G|
|Rat IL13RA1 ORF mammalian expression plasmid, N-Flag tag||RG80186-NF|
|Rat IL13RA1 ORF mammalian expression plasmid, N-His tag||RG80186-NH|
|Rat IL13RA1 ORF mammalian expression plasmid, N-Myc tag||RG80186-NM|
|Rat IL13RA1 ORF mammalian expression plasmid, N-HA tag||RG80186-NY|
|Rat IL13RA1 natural ORF mammalian expression plasmid||RG80186-UT|
|Learn more about expression Vectors|
Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.