|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD208 ORF mammalian expression plasmid, C-GFPSpark tag||HG10527-ACG|
|Human CD208 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10527-ACR|
|Human CD208 ORF mammalian expression plasmid, C-Flag tag||HG10527-CF|
|Human CD208 ORF mammalian expression plasmid, C-His tag||HG10527-CH|
|Human CD208 ORF mammalian expression plasmid, C-Myc tag||HG10527-CM|
|Human CD208 ORF mammalian expression plasmid, C-HA tag||HG10527-CY|
|Human CD208 Gene cDNA clone plasmid||HG10527-M|
|Human CD208 ORF mammalian expression plasmid, Flag tag||HG10527-M-F|
|Human CD208 ORF mammalian expression plasmid, N-Flag tag||HG10527-NF|
|Human CD208 ORF mammalian expression plasmid, N-His tag||HG10527-NH|
|Human CD208 ORF mammalian expression plasmid, N-Myc tag||HG10527-NM|
|Human CD208 ORF mammalian expression plasmid, N-HA tag||HG10527-NY|
|Human CD208 natural ORF mammalian expression plasmid||HG10527-UT|
|Learn more about expression Vectors|
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.