|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LBP ORF mammalian expression plasmid, C-GFPSpark tag||HG10526-ACG|
|Human LBP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10526-ACR|
|Human LBP ORF mammalian expression plasmid, C-Flag tag||HG10526-CF|
|Human LBP ORF mammalian expression plasmid, C-His tag||HG10526-CH|
|Human LBP ORF mammalian expression plasmid, C-Myc tag||HG10526-CM|
|Human LBP ORF mammalian expression plasmid, C-HA tag||HG10526-CY|
|Human LBP Gene cDNA clone plasmid||HG10526-M|
|Human LBP ORF mammalian expression plasmid, Flag tag||HG10526-M-F|
|Human LBP ORF mammalian expression plasmid, N-Flag tag||HG10526-NF|
|Human LBP ORF mammalian expression plasmid, N-His tag||HG10526-NH|
|Human LBP ORF mammalian expression plasmid, N-Myc tag||HG10526-NM|
|Human LBP ORF mammalian expression plasmid, N-HA tag||HG10526-NY|
|Human LBP natural ORF mammalian expression plasmid||HG10526-UT|
|Learn more about expression Vectors|
Lipopolysaccharide binding protein ( LBP ) is a glycoprotein that is synthesized principally by hepatocytes. LBP is a trace plasma protein that binds to the lipid A moiety of bacterial lipopolysaccharides ( LPSs ). LBP binds directly to the outer membrane of Gram-negative bacteria and to purified aggregates of extracted endotoxin, and catalyses the delivery of endotoxin to membrane ( mCD14,GPI-Linked ) and soluble ( sCD14 ) forms of CD14, thereby markedly increasing host cell sensitivity to endotoxin. LBP efficiently catalyses the transfer of individual molecules of endotoxin to (s)CD14 only when LBP–endotoxin aggregates are formed in the presence of albumin. In the presence of EDTA, LBP binding promotes further disaggregation of endotoxin. LBP binding does not have such drastic effects under more physiological conditions, but may still induce more subtle topological rearrangements of endotoxin.