|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TREM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10511-ACG|
|Human TREM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10511-ACR|
|Human TREM1 ORF mammalian expression plasmid, C-Flag tag||HG10511-CF|
|Human TREM1 ORF mammalian expression plasmid, C-His tag||HG10511-CH|
|Human TREM1 ORF mammalian expression plasmid, C-Myc tag||HG10511-CM|
|Human TREM1 ORF mammalian expression plasmid, C-HA tag||HG10511-CY|
|Human TREM1 Gene cDNA clone plasmid||HG10511-M|
|Human TREM1 ORF mammalian expression plasmid, N-Flag tag||HG10511-NF|
|Human TREM1 ORF mammalian expression plasmid, N-His tag||HG10511-NH|
|Human TREM1 ORF mammalian expression plasmid, N-Myc tag||HG10511-NM|
|Human TREM1 ORF mammalian expression plasmid, N-HA tag||HG10511-NY|
|Human TREM1 natural ORF mammalian expression plasmid||HG10511-UT|
|Learn more about expression Vectors|
TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-α and IL-1β, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP)-1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-γ. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.