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CD23 / FCER2 Antibody, Mouse MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
CD23Antibody Product Information
Immunogen:Recombinant Human CD23 / FCER2 protein (Catalog#10261-H07H)
Clone ID:05
Ig Type:Mouse IgG2a
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD23 / FCER2 (rh CD23 / FCER2; Catalog#10261-H07H; P06734-1; Asp 48-Ser 321). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD23Antibody Usage Guide
Specificity:Human CD23 / FCER2
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD23. The detection limit for Human CD23 is approximately 0.0195 ng/well.

IHC-P: 5-20 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
CD23 / FCER2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD23 in human spleen with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
CD23 / FCER2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD23 in human tonsil with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
Background

Fc fragment of IgE, low affinity II, receptor for (CD23) or CD23 antigen is a member of the cluster of differentiation family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling). Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD23/FCER2 is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Increased levels of soluble CD23/FCER2 cause the recruitment of non-sensitised B-cells in the presentation of antigen peptides to allergen-specific B-cells, therefore increasing the production of allergen specific IgE. IgE, in turn, is known to upregulate the cellular expression of CD23 and Fc epsilon RI (high-affinity IgE receptor).

References
  • Aubry JP, et al. (1992) CD21 is a ligand for CD23 and regulates IgE production. Nature. 358(6386): 505-7.
  • Punnonen J, et al. (1993) Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells. Proc Natl Acad Sci U S A. 90(8): 3730-4.
  • Yu P, et al. (1994) Negative feedback regulation of IgE synthesis by murine CD23. Nature. 369(6483): 753-6.
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