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Human CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated

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Human CD32b Protein Product Information
Synonym:CD32, CD32B, FCG2, FCGR2, FCGR2B, IGFR2
Protein Construction:A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CD32b Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA.
Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b(Cat:10259-H27H-B). The EC50 of Human CD32b(Cat:10259-H27H-B) is 1.6 - 3.7 μg/ml.
2. Labeling ratio of biotin to protein: 0.5
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ala 46
Molecule Mass:The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CD32b Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CD32b Protein SDS-PAGE
Human CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated SDS-PAGE
Other CD32b Recombinant Protein Products
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Catalog: 10259-H27H-B-50
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