|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BCL2L1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10455-ACG|
|Human BCL2L1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10455-ACR|
|Human BCL2L1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10455-ANG|
|Human BCL2L1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10455-ANR|
|Human BCL2L1 ORF mammalian expression plasmid, C-Flag tag||HG10455-CF|
|Human BCL2L1 ORF mammalian expression plasmid, C-His tag||HG10455-CH|
|Human BCL2L1 ORF mammalian expression plasmid, C-Myc tag||HG10455-CM|
|Human BCL2L1 ORF mammalian expression plasmid, C-HA tag||HG10455-CY|
|Human BCL2L1 Gene cDNA clone plasmid||HG10455-M|
|Human BCL2L1 natural ORF mammalian expression plasmid||HG10455-M-N|
|Human BCL2L1 ORF mammalian expression plasmid, N-Flag tag||HG10455-NF|
|Human BCL2L1 ORF mammalian expression plasmid, N-His tag||HG10455-NH|
|Human BCL2L1 ORF mammalian expression plasmid, N-Myc tag||HG10455-NM|
|Human BCL2L1 ORF mammalian expression plasmid, N-HA tag||HG10455-NY|
|Human BCL2L1 natural ORF mammalian expression plasmid||HG10455-UT|
|Learn more about expression Vectors|
B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.