|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human OSM ORF mammalian expression plasmid, C-GFPSpark tag||HG10452-ACG|
|Human OSM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10452-ACR|
|Human OSM ORF mammalian expression plasmid, C-Flag tag||HG10452-CF|
|Human OSM ORF mammalian expression plasmid, C-His tag||HG10452-CH|
|Human OSM ORF mammalian expression plasmid, C-Myc tag||HG10452-CM|
|Human OSM ORF mammalian expression plasmid, C-HA tag||HG10452-CY|
|Human OSM Gene cDNA clone plasmid||HG10452-M|
|Human OSM natural ORF mammalian expression plasmid||HG10452-M-N|
|Human OSM ORF mammalian expression plasmid, N-Flag tag||HG10452-NF|
|Human OSM ORF mammalian expression plasmid, N-His tag||HG10452-NH|
|Human OSM ORF mammalian expression plasmid, N-Myc tag||HG10452-NM|
|Human OSM ORF mammalian expression plasmid, N-HA tag||HG10452-NY|
|Human OSM natural ORF mammalian expression plasmid||HG10452-UT|
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Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many biometabolism processes including liver development, haematopoeisis, inflammation, bone formation and destruction and possibly CNS development. Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-teminal most 31 amino acids produces the fully active 196 residue form.