|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ARSA ORF mammalian expression plasmid, C-GFPSpark tag||HG10449-ACG|
|Human ARSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10449-ACR|
|Human ARSA ORF mammalian expression plasmid, C-Flag tag||HG10449-CF|
|Human ARSA ORF mammalian expression plasmid, C-His tag||HG10449-CH|
|Human ARSA ORF mammalian expression plasmid, C-Myc tag||HG10449-CM|
|Human ARSA ORF mammalian expression plasmid, C-HA tag||HG10449-CY|
|Human ARSA Gene cDNA clone plasmid||HG10449-M|
|Human ARSA ORF mammalian expression plasmid, Flag tag||HG10449-M-F|
|Human ARSA natural ORF mammalian expression plasmid||HG10449-M-N|
|Human ARSA ORF mammalian expression plasmid, N-Flag tag||HG10449-NF|
|Human ARSA ORF mammalian expression plasmid, N-His tag||HG10449-NH|
|Human ARSA ORF mammalian expression plasmid, N-Myc tag||HG10449-NM|
|Human ARSA ORF mammalian expression plasmid, N-HA tag||HG10449-NY|
|Human ARSA natural ORF mammalian expression plasmid||HG10449-UT|
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Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.