After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human LDLR / LDL Receptor Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human LDLR Protein Product Information
Protein Construction:A DNA sequence encoding the extracellular domain of human LDLR (NP_000518.1) precursor (Met 1-Arg 788) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human LDLR Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:Measure by its ability to bind with human PCSK9 in a functional ELISA .
1. Immobilized human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR . The EC50 of biotinylated human LDLR is 0.61 μg/ml.
2. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR . The EC50 of biotinylated human LDLR is 0.12 μg/ml .
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ala 22
Molecule Mass:The secreted recombinant human LDLR comprises 777 amino acids with a predicted molecular mass of 86 kDa. As a result of different glycosylation, it migarates with the apparent molecular mass of 110-140 kDa in SDS-PAGE under reducing conditions corresponding to the mature and immature form.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human LDLR Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other LDLR Recombinant Protein Products
LDL Receptor Background

LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

Human LDL Receptor References
  • Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
  • Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
  • Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
  • Product nameProduct name
    Size / Price
    Catalog: 10231-H08H-20
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions