Quick Order

Text Size:AAA

Cadherin-4 / CDH4 / R-CAD Antibody, Rabbit PAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
CDH4Antibody Product Information
Immunogen:Recombinant Human CDH4 protein (Catalog#10230-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CDH4 extracellular domain (rh CDH4; Catalog#10230-H08H; NP_001785.2; Met 1-Ala 734). Total IgG was purified by Protein A affinity chromatography.
CDH4Antibody Usage Guide
Specificity:Human Cadherin-4 / CDH4
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CDH4. The detection limit for Human CDH4 is 0.00245 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

The cadherin superfamily is a large family that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events, and can be divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains, that is: classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules. Human cadherin 4, type 1, R-cadherin (retinal), also known as CDH4, CAD4 and RCAD, is a classical cadherin from the cadherin superfamily. It is a calcium-dependent adhesion molecule and a type I  transmembrane glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. CDH4 is thought to play an important role during brain segmentation and neuronal outgrowth, and also exerts critical actions in kidney and muscle development. CDH4 is expressed in vascular smooth muscle, pancreatic β-cells, thyroid follicular cells, sensory neurons of the dorsal root ganglia, and, possibly, astrocytes and endothelium of the retina. As a classic cadherin, CDH4 forms both homodimers and heterodimers with N-cadherin. The extracellular region of human CDH4 is 96% aa identical to that of mouse CDH4.

References
  • Suzuki, S. et al., 1991, Cell Regul. 2: 261-270.
  • Matsunami, H. et al., 1993, J. Cell Sci. 106: 401-409.
  • Tanihara, H. et al.,1995, Cell Adhes. Commun. 2 (1): 15-26.
  • Shan, W-S., et al., 2000, J. Cell Biol. 148: 579-590.
  • Fagman, H. et al, 2003, Endocrinology. 144: 3618-3624.
  • Koch, A.W. et al., 2004, Cell. Mol. Life Sci. 61: 1884-1895.
  • Related Products
    Product nameProduct name
    Size / Price
    Catalog:10230-RP01-100
    List Price: $135.00  (Save $0.00)
    Price:$135.00      [How to order]
    AvailabilityIn Stock
    Images
      Recently Viewed Items
        Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"