After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human R-Cadherin / CDH4 Protein (His Tag)

DatasheetReviewsRelated ProductsProtocols
Human CDH4 Protein Product Information
Synonym:CDH4, CAD4, FLJ22202, FLJ40547, MGC126700, MGC138355, RCAD
Protein Construction:A DNA sequence encoding the extracellular domain of human CAD4 (NP_001785.2) (Met 1-Ala 734) was expressed with a fused polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CDH4 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells . Human CAD4 immobilized at 0.8 μg/ml, 100 μl/well will mediate >20% C6 cell adhesion.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His 21
Molecule Mass:The recombinant pro form of human CAD4 comprises 725 amino acids and predicts a molecular mass of 80 kDa. As a result of glycosylation, rhCAD4 migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CDH4 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CDH4 Protein SDS-PAGE
Human R-Cadherin / CDH4 Protein (His Tag) SDS-PAGE
Other CDH4 Recombinant Protein Products
R-Cadherin / CDH4 Background

The cadherin superfamily is a large family that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events, and can be divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains, that is: classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules. Human cadherin 4, type 1, R-cadherin (retinal), also known as CDH4, CAD4 and RCAD, is a classical cadherin from the cadherin superfamily. It is a calcium-dependent adhesion molecule and a type I  transmembrane glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. CDH4 is thought to play an important role during brain segmentation and neuronal outgrowth, and also exerts critical actions in kidney and muscle development. CDH4 is expressed in vascular smooth muscle, pancreatic β-cells, thyroid follicular cells, sensory neurons of the dorsal root ganglia, and, possibly, astrocytes and endothelium of the retina. As a classic cadherin, CDH4 forms both homodimers and heterodimers with N-cadherin. The extracellular region of human CDH4 is 96% aa identical to that of mouse CDH4.

Human R-Cadherin / CDH4 References
  • Suzuki, S. et al., 1991, Cell Regul. 2: 261-270.
  • Matsunami, H. et al., 1993, J. Cell Sci. 106: 401-409.
  • Tanihara, H. et al.,1995, Cell Adhes. Commun. 2 (1): 15-26.
  • Shan, W-S., et al., 2000, J. Cell Biol. 148: 579-590.
  • Fagman, H. et al, 2003, Endocrinology. 144: 3618-3624.
  • Koch, A.W. et al., 2004, Cell. Mol. Life Sci. 61: 1884-1895.
  • Product nameProduct name
    Size / Price
    Catalog: 10230-H08H-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.