|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IFNW1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10353-ACG|
|Human IFNW1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10353-ACR|
|Human IFNW1 ORF mammalian expression plasmid, C-Flag tag||HG10353-CF|
|Human IFNW1 ORF mammalian expression plasmid, C-His tag||HG10353-CH|
|Human IFNW1 ORF mammalian expression plasmid, C-Myc tag||HG10353-CM|
|Human IFNW1 ORF mammalian expression plasmid, C-HA tag||HG10353-CY|
|Human IFNW1 Gene cDNA clone plasmid||HG10353-M|
|Human IFNW1 ORF mammalian expression plasmid, Flag tag||HG10353-M-F|
|Human IFNW1 ORF mammalian expression plasmid, N-Flag tag||HG10353-NF|
|Human IFNW1 ORF mammalian expression plasmid, N-His tag||HG10353-NH|
|Human IFNW1 ORF mammalian expression plasmid, N-Myc tag||HG10353-NM|
|Human IFNW1 ORF mammalian expression plasmid, N-HA tag||HG10353-NY|
|Human IFNW1 natural ORF mammalian expression plasmid||HG10353-UT|
|Learn more about expression Vectors|
IFNs are a large family of proteins having antiviral, antiproliferative, and immunomodulatory effects, and are divided into two major classes, type I and type I I, on the basis of differences in receptor binding and nucleotide sequence. Type I IFNs consist of IFN α, β, τ, and ω and bind to the type I IFN receptor, whereas IFN-γ is the only type I I IFN and is specific for the type I I IFN receptor. Human IFN-ω, was identified by three independent groups in 1985 and is structurally related to IFN-α and -β. Both human IFN-ω and IFN-α are produced by virally induced leukocytes and have similar antiviral activities on human cell lines, and a sizeable proportion (at least 10%) of the total antiviral activity of leukocyte IFN is contributed by IFN-ωl. In addition, it was reported that IFN-ω could inhibit the growth of human tumors in vivo.