|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Mouse CTSA ORF mammalian expression plasmid, C-GFPSpark tag||MG50348-ACG|
|Mouse CTSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50348-ACR|
|Mouse CTSA ORF mammalian expression plasmid, C-Flag tag||MG50348-CF|
|Mouse CTSA ORF mammalian expression plasmid, C-His tag||MG50348-CH|
|Mouse CTSA ORF mammalian expression plasmid, C-Myc tag||MG50348-CM|
|Mouse CTSA ORF mammalian expression plasmid, C-HA tag||MG50348-CY|
|Mouse CTSA Gene cDNA clone plasmid||MG50348-M|
|Mouse CTSA ORF mammalian expression plasmid, N-Flag tag||MG50348-NF|
|Mouse CTSA ORF mammalian expression plasmid, N-His tag||MG50348-NH|
|Mouse CTSA ORF mammalian expression plasmid, N-Myc tag||MG50348-NM|
|Mouse CTSA ORF mammalian expression plasmid, N-HA tag||MG50348-NY|
|Mouse CTSA natural ORF mammalian expression plasmid||MG50348-UT|
|Learn more about expression Vectors|
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.