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E-Cadherin / CDH1 / E-cad / CD324 Antibody, Mouse MAb

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CDH1Antibody Product Information
Immunogen:Recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin protein (Catalog#10204-H08H)
Clone ID:7G5D4
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CDH1Antibody Usage Guide
Specificity:Human Cadherin-1 / CDH1 / CD324 / E-cadherin
Application:ELISA, ICC/IF, IF

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human E-cad.

ICC/IF: 15-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of human E-cadherin in human embryonic stem (ES) cells with mouse monoclonal antibody (15 μg/mL). The image showing membrane staining of human ES cells.
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of human E-cadherin in MCF-7 cells with mouse monoclonal antibody (15 µg/mL). The image showing membrane staining of MCF-7 cells.
Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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    • E-Cadherin / CDH1 / E-cad / CD324 Antibody, Mouse MAb, Immunofluorescence
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