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Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (Fc Tag)

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CMV gB Protein Product Information
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
CMV gB Protein QC Testing
Purity:> 80 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to bind biotinylated Human CD209-Fc (Cat:10200-H01H) in functional ELISA.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Asn 25
Molecule Mass:The recombinant human CMV glycoprotein B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB/Fc monomer is approximately 160-170 kDa due to glycosylation.
Formulation:Lyophilized from sterile 100mM Gly, 10mM NaCl, 50mM Tris, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
CMV gB Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CMV Glycoprotein B / gB Protein (Fc Tag) SDS-PAGE
CMV Glycoprotein B / gB Protein (Fc Tag) SDS-PAGE
Other gB Recombinant Protein Products
Cytomegalovirus Glycoprotein B Background

Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 907-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.

CMV Cytomegalovirus Glycoprotein B References
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.

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    Catalog: 10202-V02H1-20
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