|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GranzymeH ORF mammalian expression plasmid, C-GFPSpark tag||HG10348-ACG|
|Human GranzymeH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10348-ACR|
|Human GranzymeH ORF mammalian expression plasmid, C-Flag tag||HG10348-CF|
|Human GranzymeH ORF mammalian expression plasmid, C-His tag||HG10348-CH|
|Human GranzymeH ORF mammalian expression plasmid, C-Myc tag||HG10348-CM|
|Human GranzymeH ORF mammalian expression plasmid, C-HA tag||HG10348-CY|
|Human GranzymeH Gene cDNA clone plasmid||HG10348-M|
|Human GranzymeH ORF mammalian expression plasmid, Flag tag||HG10348-M-F|
|Human GranzymeH ORF mammalian expression plasmid, N-Flag tag||HG10348-NF|
|Human GranzymeH ORF mammalian expression plasmid, N-His tag||HG10348-NH|
|Human GranzymeH ORF mammalian expression plasmid, N-Myc tag||HG10348-NM|
|Human GranzymeH ORF mammalian expression plasmid, N-HA tag||HG10348-NY|
|Human GranzymeH natural ORF mammalian expression plasmid||HG10348-UT|
|Learn more about expression Vectors|
Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural?killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.