|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-GFPSpark tag||HG10346-ACG|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10346-ACR|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-Flag tag||HG10346-CF|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-His tag||HG10346-CH|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-Myc tag||HG10346-CM|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, C-HA tag||HG10346-CY|
|Human ICAM-1 / CD54 Gene cDNA clone plasmid||HG10346-M|
|Human ICAM-1 / CD54 natural ORF mammalian expression plasmid||HG10346-M-N|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, N-Flag tag||HG10346-NF|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, N-His tag||HG10346-NH|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, N-Myc tag||HG10346-NM|
|Human ICAM-1 / CD54 ORF mammalian expression plasmid, N-HA tag||HG10346-NY|
|Human ICAM-1 / CD54 natural ORF mammalian expression plasmid||HG10346-UT|
|Learn more about expression Vectors|
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.