|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GranzymeB ORF mammalian expression plasmid, C-GFPSpark tag||HG10345-ACG|
|Human GranzymeB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10345-ACR|
|Human GranzymeB ORF mammalian expression plasmid, C-Flag tag||HG10345-CF|
|Human GranzymeB ORF mammalian expression plasmid, C-His tag||HG10345-CH|
|Human GranzymeB ORF mammalian expression plasmid, C-Myc tag||HG10345-CM|
|Human GranzymeB ORF mammalian expression plasmid, C-HA tag||HG10345-CY|
|Human GranzymeB Gene cDNA clone plasmid||HG10345-M|
|Human GranzymeB ORF mammalian expression plasmid, Flag tag||HG10345-M-F|
|Human GranzymeB ORF mammalian expression plasmid, N-Flag tag||HG10345-NF|
|Human GranzymeB ORF mammalian expression plasmid, N-His tag||HG10345-NH|
|Human GranzymeB ORF mammalian expression plasmid, N-Myc tag||HG10345-NM|
|Human GranzymeB ORF mammalian expression plasmid, N-HA tag||HG10345-NY|
|Human GranzymeB natural ORF mammalian expression plasmid||HG10345-UT|
|Learn more about expression Vectors|
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.