|Datasheet||Specific References||Reviews||Related Products||Protocols|
|JAM, FA11R, KAT, JAM1, JAMA, JCAM, CD321, JAM-1, JAM-A, PAM-1|
|A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with a polyhistidine tag at the C-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 97 % as determined by SDS-PAGE|
|Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.|
When 8 x 104 cells/well are added to JAM-A-Fc coated plates (2.5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The secreted recombinant human JAM-A comprises 227 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, it migrates with the molecular mass of approximately 28-32 kDa in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.