|Datasheet||Specific References||Reviews||Related Products||Protocols|
|EFNA3, EFL2, EPLG3, Ehk1-L, LERK3|
|A DNA sequence encoding the human EphrinA3 (NP_004943.1) (Met 1-Ser 213) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 70 % as determined by SDS-PAGE|
|Measured by its ability to bind biotinylated mouse EPHA6-Fc (Cat:50630-M02H) in a functional ELISA.|
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant human EphrinA3 consists of 197 amino acids and has a predicted molecular mass of 22 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3 is approximately 35-40 kDa due to glycosylation.|
|Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.