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Human NBL1 / DAND1 / DAN Protein (His Tag)

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Human NBL1/DAN Protein Product Information
Synonym:RP5-1056L3.4, D1S1733E, DAN, DAND1, MGC8972, NB, NO3, NBL-1.
Protein Construction:A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human NBL1/DAN Protein QC Testing
Purity:> 98 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to inhibit BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ala 16
Molecule Mass:The recombinant human DAN consists of 176 amino acids and has a calculated molecular mass of 19.3 kDa. As a result of glycosylation, rh DAN migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human NBL1/DAN Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human NBL1/DAN Protein SDS-PAGE
Human NBL1 / DAND1 / DAN Protein (His Tag) SDS-PAGE
Other NBL1/DAN Recombinant Protein Products
NBL1/DAND1 Background

The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.

Human NBL1/DAND1 References
  • Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
  • Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
  • Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
  • Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.
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    Catalog: 10169-H08H-50
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.