|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human OMG / OMGP ORF mammalian expression plasmid, C-GFPSpark tag||HG10269-ACG|
|Human OMG / OMGP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10269-ACR|
|Human OMG / OMGP ORF mammalian expression plasmid, C-Flag tag||HG10269-CF|
|Human OMG / OMGP ORF mammalian expression plasmid, C-His tag||HG10269-CH|
|Human OMG / OMGP ORF mammalian expression plasmid, C-Myc tag||HG10269-CM|
|Human OMG / OMGP ORF mammalian expression plasmid, C-HA tag||HG10269-CY|
|Human OMG / OMGP Gene cDNA clone plasmid||HG10269-M|
|Human OMG / OMGP ORF mammalian expression plasmid, N-Flag tag||HG10269-NF|
|Human OMG / OMGP ORF mammalian expression plasmid, N-His tag||HG10269-NH|
|Human OMG / OMGP ORF mammalian expression plasmid, N-Myc tag||HG10269-NM|
|Human OMG / OMGP ORF mammalian expression plasmid, N-HA tag||HG10269-NY|
|Human OMG / OMGP natural ORF mammalian expression plasmid||HG10269-UT|
|Learn more about expression Vectors|
Mouse oligodendrocyte-myelin glycoprotein, also known as OMG and OMGP, is a cell membrane protein which contains eight LRR (leucine-rich) repeats. OMG / OMGP is a glycosylphosphatidylinositol-anchored protein expressed by neurons and oligodendrocytes in the central nervous system (CNS). OMG / OMGP is a cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. OMG / OMGP play roles in both the developing and adult central nervous system. OMG / OMGP participats in growth cone collapse and inhibition of neurite outgrowth through its interaction with NgR, the receptor for Nogo. This function requires its leucine-rich repeat domain, a highly conserved region in OMgp during mammal evolution. OMG / OMGP leucine-rich repeat domain is also implicated in the inhibition of cell proliferation. OMG / OMGP may also be involved in the formation and maintenance of myelin sheaths. Cell proliferation, neuronal sprouting and myelination are crucial processes involved in brain development and regeneration after injury.