|Datasheet||Specific References||Reviews||Related Products||Protocols|
|IL1RN, ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430|
|A DNA sequence encoding the mature form of human IL1RA isoform1 (NP_776214.1) (Arg 26-Glu 177) was expressed.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 97 % as determined by SDS-PAGE|
|1. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R2-Fc (Cat:10111-H02H), The EC50 of human IL1R2-Fc (Cat:10111-H02H) is 0.04-0.1 μg/mL.|
2. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R1-Fc (Cat:10126-H02H), The EC50 of human IL1R1-Fc (Cat:10126-H02H) is 0.08-0.2 μg/mL.
|Please contact us for more information.|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant human IL1RA consists of 153 amino acids and predicts a molecular mass of 17.3 kDa which is also estimated by SDS-PAGE.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.