|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Mouse MCPT1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50220-ACG|
|Mouse MCPT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50220-ACR|
|Mouse MCPT1 ORF mammalian expression plasmid, C-Flag tag||MG50220-CF|
|Mouse MCPT1 ORF mammalian expression plasmid, C-His tag||MG50220-CH|
|Mouse MCPT1 ORF mammalian expression plasmid, C-Myc tag||MG50220-CM|
|Mouse MCPT1 ORF mammalian expression plasmid, C-HA tag||MG50220-CY|
|Mouse MCPT1 Gene cDNA clone plasmid||MG50220-M|
|Mouse MCPT1 ORF mammalian expression plasmid, N-Flag tag||MG50220-NF|
|Mouse MCPT1 ORF mammalian expression plasmid, N-His tag||MG50220-NH|
|Mouse MCPT1 ORF mammalian expression plasmid, N-Myc tag||MG50220-NM|
|Mouse MCPT1 ORF mammalian expression plasmid, N-HA tag||MG50220-NY|
|Mouse MCPT1 natural ORF mammalian expression plasmid||MG50220-UT|
|Learn more about expression Vectors|
Mouse Mast Cell Protease 1 (MMCP-1), also known as MCP-1, MCPT-1 and β-chymase, is a member of the Chymase family of chymotrypsin-like serine proteases. MCPT-1 is a 26 kDa β-chymase that is a component of mast cell granules. It is a 226 amino acid (aa) protein that has a conserved pattern of six cysteines and one potential glycosylation site. The granule-derived mouse mast cell proteases-1 and -2 (mMCP-1 and -2) colocalize in similar quantities in mucosal mast cells but micrograms of mMCP-1 compared with nanograms of mMCP-2 are detected in peripheral blood during intestinal nematode infection. mMCP-1 isolated from serum is complexed with serpins and both the accumulation and the longevity of mMCP-1 in blood is due to complex formation, protecting it from a pathway that rapidly clears mMCP-2, which is unable to form complexes with serpins. The mucosal mast cell (MMC) granule-specific beta-chymase, mouse mast cell protease-1 (mMCP-1), is released systemically into the bloodstream early in nematode infection before parasite-specific IgE responses develop and TGF-beta1 induces constitutive release of mMCP-1 by homologues of MMC in vitro. Expression of mMCP-1 is largely restricted to intraepithelial MMC and is thought to play a role in the regulation of epithelial permeability. Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C). MCPT-1 is upregulated in the intestine in response to nematode infection, or in systemic mucosa in response to anaphylaxis. Like human α-chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure. The intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1.