|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human KIRREL ORF mammalian expression plasmid, C-GFPSpark tag||HG15752-ACG|
|Human KIRREL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15752-ACR|
|Human KIRREL ORF mammalian expression plasmid, C-Flag tag||HG15752-CF|
|Human KIRREL ORF mammalian expression plasmid, C-His tag||HG15752-CH|
|Human KIRREL ORF mammalian expression plasmid, C-Myc tag||HG15752-CM|
|Human KIRREL ORF mammalian expression plasmid, C-HA tag||HG15752-CY|
|Human KIRREL Gene cDNA clone plasmid||HG15752-G|
|Human KIRREL ORF mammalian expression plasmid, N-Flag tag||HG15752-NF|
|Human KIRREL ORF mammalian expression plasmid, N-His tag||HG15752-NH|
|Human KIRREL ORF mammalian expression plasmid, N-Myc tag||HG15752-NM|
|Human KIRREL ORF mammalian expression plasmid, N-HA tag||HG15752-NY|
|Human KIRREL natural ORF mammalian expression plasmid||HG15752-UT|
|Learn more about expression Vectors|
NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.