|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CREB1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51953-ACG|
|Mouse CREB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51953-ACR|
|Mouse CREB1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51953-ANG|
|Mouse CREB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51953-ANR|
|Mouse CREB1 ORF mammalian expression plasmid, C-Flag tag||MG51953-CF|
|Mouse CREB1 ORF mammalian expression plasmid, C-His tag||MG51953-CH|
|Mouse CREB1 ORF mammalian expression plasmid, C-Myc tag||MG51953-CM|
|Mouse CREB1 ORF mammalian expression plasmid, C-HA tag||MG51953-CY|
|Mouse CREB1 Gene cDNA clone plasmid||MG51953-G|
|Mouse CREB1 ORF mammalian expression plasmid, N-Flag tag||MG51953-NF|
|Mouse CREB1 ORF mammalian expression plasmid, N-His tag||MG51953-NH|
|Mouse CREB1 ORF mammalian expression plasmid, N-Myc tag||MG51953-NM|
|Mouse CREB1 ORF mammalian expression plasmid, N-HA tag||MG51953-NY|
|Mouse CREB1 natural ORF mammalian expression plasmid||MG51953-UT|
|Learn more about expression Vectors|
CAMP responsive element binding protein 1, also known as CREB-1, plays multiple functions as a transcription factor in gene regulation. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators.The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB-1 has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. CREB-1 is important for serotonin (5-HT)-induced long-term facilitation (LTF) of the sensorimotor synapse in Aplysia.