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Human Glypican 5 / GPC5 Protein (His Tag)

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Human Glypican 5 Protein Product Information
Synonym:GPC5, bA93M14.1, glypican proteoglycan 5, glypican-5
Protein Construction:A DNA sequence encoding the human GPC5 (NP_004457.1) (Met 1-Thr 554) was fused with a polyhistidine tag at the C-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human Glypican 5 Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA . Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml .
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Glu 25
Molecule Mass:The secreted recombinant human GPC5 consists of 540 amino acids and predicts a molecular mass of 60.5 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human Glypican 5 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other Glypican 5 Recombinant Protein Products
Glypican 5/GPC5 Background

Glypican-5 (GPC5), is a cell membrane protein which belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in vertebrates. In adult, Glypican-5 is primarily expressed in the brain. It is also detected in fetal brain, lung and liver. Glypican-5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 is over-expressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to development and/or progression of lymphomas and other tumors.

Human Glypican 5/GPC5 References
  • Yu W, et al. (2003) GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J Hum Genet. 48(6): 331-5.
  • Williamson D, et al. (2007) Role for amplification and expression of glypican-5 in rhabdomyosarcoma. Cancer Res. 67(1): 57-65.
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    Catalog: 10079-H08B-50
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