|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MST4 ORF mammalian expression plasmid, C-GFPSpark tag||MG51088-ACG|
|Mouse MST4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51088-ACR|
|Mouse MST4 ORF mammalian expression plasmid, N-GFPSpark tag||MG51088-ANG|
|Mouse MST4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51088-ANR|
|Mouse MST4 ORF mammalian expression plasmid, C-Flag tag||MG51088-CF|
|Mouse MST4 ORF mammalian expression plasmid, C-His tag||MG51088-CH|
|Mouse MST4 ORF mammalian expression plasmid, C-Myc tag||MG51088-CM|
|Mouse MST4 ORF mammalian expression plasmid, C-HA tag||MG51088-CY|
|Mouse MST4 Gene cDNA clone plasmid||MG51088-G|
|Mouse MST4 ORF mammalian expression plasmid, N-Flag tag||MG51088-NF|
|Mouse MST4 ORF mammalian expression plasmid, N-His tag||MG51088-NH|
|Mouse MST4 ORF mammalian expression plasmid, N-Myc tag||MG51088-NM|
|Mouse MST4 ORF mammalian expression plasmid, N-HA tag||MG51088-NY|
|Mouse MST4 natural ORF mammalian expression plasmid||MG51088-UT|
|Learn more about expression Vectors|
MST4, also known as mammalian STE20-like protein kinase 4, is a novel member of the germinal center kinase subfamily of human Ste20-like kinases and is closely related to MST3. The 416 amino acid full-length MST4 contains a C-terminal regulatory domain and an N-terminal kinase domain, both of which are required for full activation of the kinase. MST4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 specifically activates ERK but not JNK or p38 MAPK in transient transfected cells or in stable cell lines, and thus is biologically active in the activation of MEK/ERK pathway mediating cell growth and transformation. Further, MST4 kinase activity is stimulated significantly by epidermal growth factor receptor (EGFR) ligands, which are known to promote growth of certain cancer cells. Accordingly, MST4 have a potential role in signal transduction pathways involved in cancer progression. Three alternatively spliced isoform of MST4 have been isolated, and isoform 3 lacks an exon encoding kinase domain and may function as a dominant-negative regulator of the MST4 kinase.
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