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CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified

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ANPEPAntibody Product Information
Immunogen:Recombinant Human ANPEP protein (Catalog#10051-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human ANPEP (rh ANPEP; Catalog#10051-H08H; NP_001141.2; Lys 69-Lys 967). ANPEP specific IgG was purified by human ANPEP affinity chromatography .
ANPEPAntibody Usage Guide
Specificity:Human ANPEP / CD13
Application:WB, ELISA, IHC-P, IP

WB: 1-2 μg/mL

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ANPEP. The detection limit for Human ANPEP is approximately 0.00975 ng/well.

IHC-P: 0.1-1 μg/mL

IP: 0.5-1 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ANPEP in human renal carcinoma with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ANPEP in human liver with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Background

Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

References
  • Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.
  • Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.
  • Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
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    Catalog:10051-RP02-50
    List Price: $170.00  (Save $0.00)
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    Images
    • CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
    • CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
    • CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
    • CD13 / ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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