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Human CASP3 / Caspase-3 / CASP-3 Protein (His Tag)

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Human CASP3 Protein Product Information
Synonym:Caspase3, CASP3
Protein Construction:A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.
Species:Human
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CASP3 Protein QC Testing
Purity:(48.4+48.1) % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC.
The specific activity is > 5000 pmoles/min/μg.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CASP3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other CASP3 Recombinant Protein Products
10050-H08E
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Catalog: 10050-H08E-5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"