|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FLT1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51045-ACG|
|Mouse FLT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51045-ACR|
|Mouse FLT1 ORF mammalian expression plasmid, C-Flag tag||MG51045-CF|
|Mouse FLT1 ORF mammalian expression plasmid, C-His tag||MG51045-CH|
|Mouse FLT1 ORF mammalian expression plasmid, C-Myc tag||MG51045-CM|
|Mouse FLT1 ORF mammalian expression plasmid, C-HA tag||MG51045-CY|
|Mouse FLT1 Gene cDNA clone plasmid||MG51045-G|
|Mouse FLT1 ORF mammalian expression plasmid, N-Flag tag||MG51045-NF|
|Mouse FLT1 ORF mammalian expression plasmid, N-His tag||MG51045-NH|
|Mouse FLT1 ORF mammalian expression plasmid, N-Myc tag||MG51045-NM|
|Mouse FLT1 ORF mammalian expression plasmid, N-HA tag||MG51045-NY|
|Mouse FLT1 natural ORF mammalian expression plasmid||MG51045-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.