|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Mouse LGALS1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50100-ACG|
|Mouse LGALS1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50100-ACR|
|Mouse LGALS1 ORF mammalian expression plasmid, C-Flag tag||MG50100-CF|
|Mouse LGALS1 ORF mammalian expression plasmid, C-His tag||MG50100-CH|
|Mouse LGALS1 ORF mammalian expression plasmid, C-Myc tag||MG50100-CM|
|Mouse LGALS1 ORF mammalian expression plasmid, C-HA tag||MG50100-CY|
|Mouse LGALS1 Gene cDNA clone plasmid||MG50100-M|
|Mouse LGALS1 ORF mammalian expression plasmid, N-Flag tag||MG50100-NF|
|Mouse LGALS1 ORF mammalian expression plasmid, N-His tag||MG50100-NH|
|Mouse LGALS1 ORF mammalian expression plasmid, N-Myc tag||MG50100-NM|
|Mouse LGALS1 ORF mammalian expression plasmid, N-HA tag||MG50100-NY|
|Mouse LGALS1 natural ORF mammalian expression plasmid||MG50100-UT|
|Learn more about expression Vectors|
Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.