|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A synthetic peptide corresponding to the S2 subunit of MERS-CoV (NCoV / Novel coronavirus) spike glycoprotein (S protein).|
|0.2 μm filtered solution in PBS with 5% trehalose|
|Produced in rabbits immunized with purified, a synthetic peptide corresponding to the S2 subunit of MERS-CoV (NCoV / Novel coronavirus) spike glycoprotein (S protein), and purified by antigen affinity chromatography.|
|The antibody reacts with S2 subunit of MERS-CoV (NCoV / Novel coronavirus) Spike protein (S protein) and full-length S protein|
|WB, ELISA, IHC, IF, IP
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)|
WB: 0.5-2 μg/mL
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.