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Human bFGF / FGF2 Protein

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bFGF/FGF2Protein Product Information
Synonym:FGF2, BFGF, FGFB, HBGF-2
Protein Construction:A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.
Expressed Host:E. coli
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
bFGF/FGF2Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.1 ng/ml.
Endotoxin:< 1.0 EU per mg protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human bFGF is a disulfide-linked homodimeric protein. The reduced monomer consists of 147 amino acids and has a calculated molecular mass of 16.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of bFGF is approximately 17 kDa.
Formulation:Lyophilized from sterile 20mM Tris, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
bFGF/FGF2Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

bFGF/FGF2 Protein & Antibody Products

  • Takayama S,et al. (2001) Periodontal regeneration by FGF-2 (bFGF) in primate models. J Dent Res. 80(12): 2075-9.
  • Niu YJ, et al. (2004) Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chin Med J (Engl). 117(2): 252-7.
  • Zhang Y,et al. (2004) Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chin Med J (Engl). 117(4): 601-3.
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    Size / Price
    List Price: $58.00  (Save $20.00)
    Price:$38.00      [How to order]
    AvailabilityIn Stock
    • Human bFGF / FGF2 Protein SDS-PAGE
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