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Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified

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ACTA2Antibody Product Information
Immunogen:A synthetic peptide corresponding to the N-terminus of the Human Alpha-Smooth Muscle Actin (ACTA2).
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the Human Alpha-Smooth Muscle Actin (ACTA2), and purified by antigen affinity chromatography.
ACTA2Antibody Usage Guide
Specificity:Human Alpha-Smooth Muscle Actin (ACTA2)
Application:IHC-P

IHC-P: 0.1-1 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of Human Alpha-Smooth Muscle Actin (ACTA2) in human uterus with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle cells.
Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human Actin in human small intestine with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle cells.
Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of Human Alpha-Smooth Muscle Actin (ACTA2) in human epididymis with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle cells.
Background

Actins are globular multi-functional proteins which can be detected in all eukaryotic cells. In vertebrates, there are three main groups of actins that possess slightly different functions: alpha, beta, and gamma. The alpha actins, found in muscle tissues, are a major constituent of the contractile apparatus. Beta-actin, found at the expanding edge of cells, uses the projection of its cellular structure as its mean of mobility. Gamma-actin is found in the filaments of stress fibres. ACTA2 is an alpha actin that is found in skeletal muscle. Expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.

References
  • Ueyama H, et al., 1990, Jinrui Idengaku Zasshi. 35(2): 145-50.
  • Snásel J, et al., 1997, Folia Biol. 42(5): 227-30.
  • Adams LD, et al., 1992, AIDS Res Hum. 8(2): 291-5.
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    Catalog:100125-RP02-50
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    • Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
    • Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
    • Alpha-Smooth Muscle Actin (ACTA2) Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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