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Sox2 Antibody, Mouse MAb

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Sox2Antibody Product Information
Immunogen:A synthetic peptide corresponding to the N-terminus of the Human Sox2.
Clone ID:03
Ig Type:Mouse IgG2a
Concentration:1 μg/Test
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the Human Sox2. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Sox2Antibody Usage Guide
Specificity:Human Sox2
Application:FCM
Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
Sox2 Antibody, Mouse MAb, Flow cytometric
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Caption:
Flow cytometric analysis of Sox2 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-Sox2 monoclonal antibody (1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Background

SOX2, also known as ANOP3, is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. SOX2 is critical for early embryogenesis and for embryonic stem cell pluripotency. It keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Defects in SOX2 can cause microphthalmia, syndromic, 3. A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.

References
  • Lu Y, et al., 2010, PLoS ONE. 5(6): e11022.
  • Gontan C, et al., 2008, Dev. Biol. 317 (1): 296-309.
  • Shi W, et al., 2006, J. Biol. Chem. 281 (33): 23319-25.
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    Catalog:100106-MM03-50
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    • Sox2 Antibody, Mouse MAb, Flow cytometric
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