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HER2 / ErbB2 / CD340 Antibody, Rabbit MAb

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Human ErbB2/HER2 Antibody Product Information
Immunogen:Recombinant Human ErbB2 protein (Catalog#10004-H08H)
Clone ID:002
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human ErbB2 / HER2 extracellular domain (rhErbB2; Catalog#10004-H08H; NP_004439.2; Met 1-Thr 652) .
Human ErbB2/HER2 Antibody Usage Guide
Specificity:Human ErbB2 / HER2 / CD340
No cross-reactivity in ELISA with
Human ErbB1 / EGFR
Human ErbB3 / HER3
Human ErbB4 / HER4
Application:ELISA, FCM, ICC/IF, IF

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ErbB2. The detection limit for Human ErbB2 is approximately 0.0049 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 5-20μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other ErbB2/HER2 Antibody Products
HER2/ErbB2 Background

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Human HER2/ErbB2 References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"