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EGFR / HER1 / ErbB1 Antibody, Mouse MAb

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EGFR/ErbB1/HER1Antibody Product Information
Immunogen:Recombinant Human EGFR / ErbB / HER1 Protein (Catalog#10001-H08H)
Clone ID:3D2H11C5
Ig Type:Mouse IgG2b
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / ErbB / HER1 (rh EGFR / ErbB / HER1; Catalog #10001-H08H; Met 1-Ser 645; NP_005219). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
EGFR/ErbB1/HER1Antibody Usage Guide
Specificity:Human EGFR / ErbB / HER1
No cross-reactivity in ELISA with
Human EGFR/ErbB1
Human ErbB2
Human ErbB3
Human ErbB4
Human cell lysate (293 cell line)
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.0195 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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    Catalog:10001-MM01-50
    List Price: $135.00  (Save $0.00)
    Price:$135.00      [How to order]
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