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CHO Cell Transient Transfection Service

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CHO Cell Transient Transfection Service

Host Cells: CHO Cells, Engineered Chinese Hamster Ovary Cells
Optimized for transient transfection production of proteins and antibodies
Technology: Recombinant technology, transient transfection
Stirred-tank cell culture bioreactors
Cell Culture: Serum-free CHO cell culture, fed-batch cell culture
Transfection: Highly efficient transient transfection of suspension CHO cells
Transfection reagent: Sinofection Transfection Reagent
Cell Culture Scale: High throughput small scale CHO cell culture at 200 mL to 1 L scale
2L stirred tank CHO cell culture
5L stirred tank CHO cell culture
10L stirred tank CHO cell culture
20L stirred tank CHO cell culture
50L stirred tank CHO cell culture
100L Stirred tank CHO cell culture
300L Stirred tank CHO cell culture
Service Mode: Cell culture volume based charge or deliverable based charge
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas

CHO Cell Transient Transfection Service Description

In CHO (Chinese Hamster Ovary) cell transient transfection, large quantities of DNA plasmids containing the target gene of interest are transfected into the host CHO cells, with an efficient transfection reagent. Regular CHO cells have very low transfection efficiency. Sino Biological has developed an engineered CHO cell line. The optimized CHO cells have an improved transfection efficiency and can maintain a certain number of DNA copies within the cells for a short period of time, up to 6-12 days. The advantage of transient transfection is that it totally skipped the need for lengthy and costly stable cell line development, and hence it is highly preferable for quick and high-throughput small scale protein and antibody production.

Sino Biological has also optimized the serum-free culture medium for optimal transfection efficiency and hence improved protein ..... <Learn More>