Antibody chimerization refers to changing the antibody constant region from one species to another. The early application of such a technology is the chimerization of mouse antibody into mouse-human chimeric antibody to reduce the antibody's immunogenicity and retain the ability to specifically bind to the antigen as a clinical product in human use. These chimeric antibodies generally have a longer half-life, and a greater ability to mediate complement-mediated cytotoxicity (CDC), and ADCC.
In fact, this technology can be applied in many other situations. For example, one could change a human antibody's constant region into a mouse constant gene and generate a human-mouse chimeric antibody. Similarly, one could change the constant regions from one species to another, as well as change from one subtype to another subtype within the same species. In theory, all the above stated scenario could all be possible, but practically, making it happen is not as straight and could be fairly challenging. Sino Biological has rich experience in chimeric antibody production covering not only human and mouse antibodies, but also rabbit, rat, canine antibodies. Our professional scientists and engineers are at your service to support your antibody chimerization needs.
|Gene synthesis and codon optimization (optional)||1-2 weeks||• Purified antibody
• SDS-PAGE data
|Vector construction||1-2 weeks|
|• Cloning the HC and LC gene into optimized expression vectors
• Plasmid sequencing
• Large scale plasmid production
|Expression & purification||1-2 weeks|
|• Transient transfection of HEK293 / CHO cells
• Purify the antibody
• Polishing if needed
|QC analysis||3 days|
|• Analysis by SDS-PAGE and UV
• Purity analysis by HPLC-SEC and endotoxin level analysis
• Client needs to provide the antibody sequence or plasmid.
• A standard service includes: antibody concentration detected by A280 and purity evaluated by SDS-PAGE. If other detection services such as SEC-HPLC and endotoxin test are required, please indicate it in the request form.