cDNA Clones

Sino Biological Inc. provides scientists worldwide with multiple forms of cDNA clones including expression-ready cDNA ORF clones, lentivirus cDNA ORF clones, and cDNA Clone in cloning vectors. All gene products were verified by full-length sequencing to ensure high-quality cDNA clones. Sino Biological has always been committed to providing customers with high quality products and services.

Advantages of cDNA Clones

Gene Bank:11,000+ molecules,400,000+ cDNA clones,most are ready to ship
Multiple Species:Human、Mouse、Rat、Rabbit、Rhesus、Cynomolgus、Canine、Ferret、zebra fish、Virus...
Multiple Tags:N-terminal or C-terminal Myc、HA、FLAG、His、GFPSpark® and OFPSpark®
Influenza Virus Gene Bank:60 subtypes, 160 strains, including H7N9, H5N1 and other popular strains; and genes of nearly 40 kinds of viruses such as MERS COV, HCV, RSV, HCov...
Full length sequence confirmed:Each gene product is verified by full-length sequencing; ~100% sequence accuracy
Customized service:Meet customer's needs

Types of cDNA Clone Products

cDNA Clone in Cloning Vector
Sequence Verification Full length sequence confirmed
Expression Validated No
Expression Host None
Vectors pMD18-T/pMD19-T/pGEM-T/pUC19
Tags None
Application PCR template; quantitative PCR template; study of gene function
Features Ultra low-cost
Expression-ready ORF Clone
Sequence Verification Full length sequence confirmed
Expression Validated Yes
Expression Host Mammalian cell
Vectors pCMV/pCMV3-Series
Tags untagged、N-/C-Flag/HA/His/Myc/GFPSpark®/OFPSpark®
Application Protein overexpression; protein detection and purification; cell localization; stable strain construction
Features Expression validated, quality assurance
Lentiviral ORF Clone
Sequence Verification Full length sequence confirmed
Expression Validated No
Expression Host Mammalian cell
Vectors pLV-Series
Tags untagged、N-/C-Flag/HA/His/Myc/GFPSpark/OFPSpark
Application Packaging lentivirus; protein overexpression; stable strain construction
Features Transfection of dividing and non-dividing cells, primary cells, stem cells and other difficult-to-transfect cells

Expression-ready ORF Clone: Expression Validated

Human PTPN1,N-Flag
HG10304-NF
Human IDH1,N-GFPSpark®
HG12055-ANG
Human SIRT6,C-GFPSpark®
HG12156-ACG
H7N9-NP,C-Flag
VG40110-CF
 
   
Human PKM,C-OFPSpark®
HG11430-ACR
Human MTOR,C-OFPSpark®
HG11508-ACR
   

Featured cDNA Clones:Organelle Marker Plasmid

Sino Biological provides a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular structures. The fluorescent fusion protein is introduced using a simple transfection step, after 48h, you can take the beautiful images directly by fluorescence microscopy.

GFPSpark® or OFPSpark® fluorescent colors for choice Easy labeling of cell compartments and proteins
Perfect for study of localization and trafficking No antibody or chemical treatment required

cDNA Clone Quality Control Standards

cDNA clones are controlled by strict quality control and Sino Biological only provides you with high quality plasmids:
a) One plasmid was sequenced twice to ensure sequence accuracy
b) UV and electrophoresis:A260/A280 = 1.8~2.0,guarantee plasmid purity
c) PCR identification:Identify gene size and exclude plasmid contamination

cDNA Clone Product Citations(Partial)

Organization Molecule Catalog No. Reference Citations Published Journals
Georgia Regents University, etc. ISL1 HG13122-G DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis Nat Commun
Swiss Federal
Institute of Technology
IL9 MG51302-G Glycosylation profiles determine extravasation and disease-targeting properties of armed antibodies Proc. Natl. Acad. Sci. U.S.A.
Cincinnati Children's Hospital Medical Center,etc. SMAD3 MG50991-UT TGFβ signaling inhibits goblet cell differentiation via SPDEF in conjunctival epithelium Development
INRS-Institut-Armand-Frappier BMP-4 HG10609-CF Bone morphogenetic protein 4 (BMP-4) and epidermal growth factor (EGF) inhibit metalloproteinase-9 (MMP-9) expression in cancer cells Oncoscience
Jinling Hospital, School of Medicine, Nanjing University GSK3b HG10044-M MicroRNA-451 induces epithelial–mesenchymal transition in docetaxel-resistant lung adenocarcinoma cells by targeting proto-oncogene c-Myc European Journal of Cancer
The Johns Hopkins University,etc. furin HG10141-M Preparation and properties of a papillomavirus infectious intermediate and its utility for neutralization studies Virology
Molecular Biology Service
• Molecular Biology Service
• Real Time PCR Service
• Real Time PCR Primer and Probe Design
• Real Time Quantitative PCR Analysis