CD74 Antibody

CD74 Antibody Overview

There are 11 CD74 antibodies which are validated in multiple tissues with various applications, including WB, FCM, IHC-P, ELISA, IP. There are 2 CD74 antibody for WB, 6 CD74 antibody for FCM, 3 CD74 antibody for IHC-P, 4 CD74 antibody for ELISA, 1 CD74 antibody for IP. Among all these CD74 antibodies, there are 4 anti-CD74 rabbit polyclonal antibodies , 6 anti-CD74 mouse monoclonal antibodies , 1 anti-CD74 rabbit monoclonal antibodies . All the CD74 anbodies are produced in house and all are in stock. CD74 antibody customerized service is available.

CD74 Antibody ELISA

CD74 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human CD74 extracellular domain (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln 73-Met 232). Total IgG was purified by Protein A affinity chromatography .

CD74 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD74 Antibody western blot

CD74 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse CD74, and purified by antigen affinity chromatography.

CD74 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD74 Antibody flow cytometry

CD74 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD74 (rh CD74; Catalog#11091-H07H; NP_005219; Met668-Ala1210). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD74 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD74 Antibody immunohistochemistry

CD74 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD74 (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln73-Met232).

CD74 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

CD74 Antibody immunoprecipitation

CD74 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD74 extracellular domain (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln 73-Met 232). CD74 specific IgG was purified by human CD74 affinity chromatography.

CD74 Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-2 μL/mg of lysate
Notes